Search results for "quantitative DNA methylation analysis"

showing 2 items of 2 documents

Clinical Validation of 13-gene DNA Methylation Analysis from Oral Brushing: A Non Invasive Sampling Procedure for Early Detection of Oral Squamous Ce…

2019

In a recent study our research group described a non-invasive sampling procedure based on [...]

Oncologymedicine.medical_specialtyoral brushing;oral squamous cell carcinoma; quantitative DNA methylation analysisbusiness.industryEarly detectionSampling (statistics)oral brushingoral squamous cell carcinomaInternal medicineDNA methylationMedicinequantitative DNA methylation analysisBasal cellGene DNA Methylation Analysis Oral Brushing Non Invasive Sampling Early Detection Oral Squamous Cell CarcinomabusinessNon invasive samplingGeneThe XV National and III International Congress of the Italian Society of Oral Pathology and Medicine
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Clinical validation of 13-gene DNA methylation analysis in oral brushing samples for detection of oral carcinoma: an Italian multicenter study

2021

Background The aim of this Italian multicenter study was to evaluate the diagnostic performance of a minimally invasive method for the detection of oral squamous cell carcinoma (OSCC) based on 13-gene DNA methylation analysis in oral brushing samples. Methods Oral brushing specimens were collected in 11 oral medicine centers across Italy. Twenty brushing specimens were collected by each center, 10 from patients with OSCC, and 10 from healthy volunteers. DNA methylation analysis was performed in blindness, and each sample was determined as positive or negative based on a predefined cutoff value. Results DNA amplification failed in 4 of 220 (1.8%) samples. Of the specimens derived from patien…

0301 basic medicinemedicine.medical_specialtyBisulfite sequencingalgorithm; bisulfite sequencing; diagnostic test; oral brushing; oral squamous cell carcinoma; quantitative DNA methylation analysisGastroenterologyoral brushing03 medical and health sciences0302 clinical medicineInternal medicineHealthy volunteersCarcinomamedicineHumansquantitative DNA methylation analysisMouth neoplasmalgorithmbusiness.industryDNA Methylationmedicine.diseaseDna amplificationoral squamous cell carcinomastomatognathic diseases030104 developmental biologyItalyOtorhinolaryngologyMulticenter studyHead and Neck Neoplasmsdiagnostic test030220 oncology & carcinogenesisDNA methylationCarcinoma Squamous Cellbisulfite sequencingMouth NeoplasmsbusinessOral medicine
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